Immunofluorescence localization of glycoproteins using tissue printing: detection of pistil extensin-like proteins in tobacco.

نویسندگان

  • E S Pierson
  • B H de Graaf
  • J Derksen
چکیده

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منابع مشابه

Changes of major wall polysaccharides and glycoproteins of tobacco cells in response to excess boron

Effects of excess concentrations of boron on major cell wall components of tobacco cells(Nicotiana tabacum L. cv. Burley 21) were studied. Pectin, xyloglucan, hydroxyproline-richglycoproteins (extensin), and arabinogalactan proteins were characterized. Results showedthat increased boron supply resulted in significant decrease in cell and cell wall dry weights.Also, high concentrations of boron ...

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Class III pistil-specific extensin-like proteins from tobacco have characteristics of arabinogalactan proteins.

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Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper

In soybean seed coats the accumulation of the hydroxyproline-rich glycoprotein extensin is regulated in a developmental and tissue-specific manner. The time course of appearance of extensin during seed development was studied by Western blot analysis and by immunogold-silver localization. Using these techniques extensin was first detected at 16-18 d after anthesis, increasing during development...

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Purification and characterization of a galactose-rich basic glycoprotein in tobacco.

We found a galactose-rich basic glycoprotein (GBGP) in the cell walls of cultured tobacco (Nicotiana tabacum) cells. GBGP and extensin were isolated as the major components of basic, salt-extracted cell wall glycoproteins. GBGP and extensin were separated by gel filtration in 6 M guanidine hydrochloride as 49- and 90-kD peaks, respectively, and further purified with reverse-phase chromatography...

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عنوان ژورنال:
  • BioTechniques

دوره 26 3  شماره 

صفحات  -

تاریخ انتشار 1999